This study aimed to select and identify elite strains of plant growth-promoting rhizobacteria (PGPR) among phosphate-solubilizing bacteria (PSB) conserved at the Ethiopian Biodiversity Institute. In-vitro screening for plant growth-promoting traits and abiotic stress tolerance identified 12 promising PGPR isolates. These strains exhibited phosphate solubilization (index 1.8–3.2), auxin production, nitrogen fixation, and ammonia production. All isolates showed drought tolerance, and some exhibited salt tolerance (up to 10% NaCl). 16S rDNA sequencing identified Pseudomonas frederiksbergensis, Pseudomonas fluorescens, Pseudomonas rhodesiae, Pseudomonas azotoformans, and Bacillus altitudinis. These strains have strong potential as bioinoculants for sustainable sorghum cultivation. Future greenhouse and field trials are recommended to validate their effectiveness.
identification, Sorghum, plant growth, rhizobacteria
Befekadu Teshome. Correspondent author. Ethiopian Biodiversity Institute, Microbial Biodiversity Research, Addis Ababa, Ethiopia. E-mail: Beftesh@gmail.com
Eleni Belay. Ethiopian Biodiversity Institute, Microbial Biodiversity Research, Addis Ababa, Ethiopia.
Barkelegne Mengesha. Ethiopian Biodiversity Institute, Microbial Biodiversity Research, Addis Ababa, Ethiopia.
Zerihun Tsegaye. Ethiopian Biodiversity Institute, Microbial Biodiversity Research, Addis Ababa, Ethiopia.
Edwin K. Akley. Savana Agricultural Research Institute, Soil Microbiology Department, Tamale, Ghana.
Awuku Frederick. Savana Agricultural Research Institute, Soil Microbiology Department, Tamale, Ghana.
"Author 1 conceptualized and finalized the research framework. Author 1 drafted the manuscript, and Author 1, 4 and 5 revised it. Author 1, 2, 3, 4 gathered the data, and Author 1 and 6 analyzed and interpreted the data. All authors contributed to the writing of the final version of the manuscript."
No potential conflict of interest was reported by the author(s).
This work is funded by the Bio-Bridge Initiative of the Secretariat of the Convention on the Biological Diversity.
Not Applicable
AI tools were not used in writing this paper.
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Cite this article:
Teshome, B., Belay, E., Mengesha, B., Tsegaye, Z., Akley, E.K. & Frederick, A. (2025). Selection and identification of Pseudomonas and Bacillus Rhizobacteria with Bioinoculant potential for Sorghum cultivation. International Journal of Science, Technology, Engineering and Mathematics, 5(2), 38-59. https://doi.org/10.53378/ijstem.353191
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