Acne vulgaris is a common skin infection that can affect nearly the entire population, with an incidence rate of approximately 80–100%. Such infections are often caused by the proliferation of Propionibacterium acnes and Staphylococcus epidermidis. The stem bark of kawista (Limonia acidissima Groff.) has shown significant potential to inhibit bacterial growth, particularly against P. acnes and S. epidermidis. This study aimed to evaluate the antibacterial effectiveness of kawista stem bark extract against these bacteria. The antibacterial assay was conducted using the agar diffusion method with paper discs. Phytochemical analysis revealed the presence of flavonoids, tannins, and saponins in the kawista stem bark. Antibacterial testing demonstrated significant inhibitory effects on bacterial growth. The average inhibition zone diameters produced by the extract against P. acnes were 4.085 mm at 25% concentration, 7.165 mm at 50%, and 9.946 mm at 75%. For S. epidermidis, the inhibition zones measured 6.076 mm at 25%, 6.825 mm at 50%, and 10.643 mm at 75%. These findings indicate that kawista stem bark extract possesses notable antibacterial activity and could be considered a potential candidate for the development of topical treatments to manage skin infections caused by Propionibacterium acnes and Staphylococcus epidermidis.