A module is one medium that can make students work independently. This study aimed to establish the validity of the researcher-developed module in general biology. It is anchored in the ADDIE model of instructional design which offers a structured procedure for creating instructional material. This study employed the design and development research approach to establish an empirical basis for the creation of instructional products, which in this case, are self-learning modules. Five experts were selected to evaluate the developed module based on the objectives, content, sequence, graphical presentation, evaluation activities, and consistency and got an overall mean rating of 4.73, interpreted as excellent, which means that they strongly agreed to the statements in the evaluation tool. The developed module was then revised based on the comments and suggestions of the experts. 22 Agriculture students were then asked to evaluate the revised module based on its format and content. The student respondents gave an excellent rating on the revised module with an overall mean rating of 4.9. It is recommended that the developed module be tried out by students taking general biology course to assess its effectiveness as a learning material.