This study aimed to assess the level of knowledge of Agribusiness students of Santo Tomas College of Agriculture Sciences and Technology on animal farming and to determine a basis for developing instructional materials. The study used a quantitative descriptive research method using test questionnaires to gather data from the respondents. A total of 92 students participated in the study using a purposive sampling design. The socio-demographic profiles of the students are not factors for their learning. The results further show that the students had poor to fair knowledge of livestock farming as only a few hit the passing rate of 75% and many scored less than the passing rate. The study's findings recommend developing new instructional materials, enhancing the course content, providing actual and hands-on training, encouraging students to participate in livestock farming activities, and conducting another comprehensive study to determine students’ adaptability and capability, recognize individual strengths, and pinpoint weaknesses in learning animal agriculture. These recommendations can help improve the knowledge level of undergraduate agribusiness students about livestock farming and prepare them for careers in the industry.