Infection control in emergency medical settings is a growing concern due to the frequent reuse of lifesaving equipment. Contaminated surfaces such as spine boards, C-collars, and Kendrick devices can serve as reservoirs for pathogenic bacteria like Staphylococcus aureus, posing risks to both patients and responders. This study assessed microbial contamination on emergency medical service equipment and explored a plant-based disinfectant as a novel intervention. A prevalence analysis was conducted to quantify S. aureus (cfu/mL) on spine boards, Kendrick extrication devices, and cervical collars (C-collars) used by Saint Gabriel College TechVoc students. Among the samples, the spine board exhibited the highest bacterial load (8.3 × 10⁵ cfu/mL), followed by the Kendrick device (3.4 × 10⁴ cfu/mL) and C-collar (2.4 × 10⁴ cfu/mL), likely due to variations in patient contact and environmental exposure. In response, a disinfectant spray was formulated using ethanolic extract of Eleusine indica (Paragis) in a hydroalcoholic base. Its antibacterial efficacy was tested against S. aureus using disc diffusion method. Treatments included benzalkonium chloride spray (BCS), 70% ethanol spray (70ES), Paragis spray, and the spray base as control. The Paragis spray showed a zone of inhibition (17.84 mm) statistically comparable to BCS (18.22 mm), significantly outperforming 70ES and the base. ANOVA and Scheffé post hoc test (p = 0.3199376 > 0.01) supported the comparable efficacy of the Paragis spray. These findings suggest E. indica as a promising, natural, and cost-effective alternative disinfectant for EMS equipment. Further in vivo and stability studies are recommended to support broader clinical application.