Leptospirosis, caused by Leptospira bacteria, remains a significant public health threat in disaster-prone areas like Aklan. This study assessed the level of knowledge and preventive measures toward leptospirosis among rice farmers in a barangay in Kalibo, Aklan. A quantitative correlational research design was employed, involving 185 registered rice farmers (80 males and 105 females). Data were collected through tests and structured survey questionnaires to evaluate farmers’ knowledge and practices regarding leptospirosis transmission, symptoms, complications, risk factors, and prevention. Results revealed that respondents had a high level of knowledge about leptospirosis but showed gaps regarding its causative agent and associated complications. While adherence to preventive measures was generally high, areas for improvement included the consistent use of protective equipment and implementation of effective rodent control strategies. The study highlights the need for targeted interventions and public health strategies to address leptospirosis among vulnerable populations, such as rice farmers. Findings emphasized the importance of localized, evidence-based health education and preventive programs to improve knowledge dissemination and promote sustainable practices. Despite its scope limitations, the study provides insights to inform policies that enhance leptospirosis prevention in agricultural communities.