Plastic seedling trays are widely used in agriculture and are vital for plant growth. However, improper disposal of these trays has become a significant concern, contributing to the growing issue of plastic pollution. With the increasing awareness of plastic waste and the environmental impact of conventional plastic trays, eco-friendly alternatives are in high demand. This study investigates the viability of Malunggay as a cellulose source to produce cellulose-based bio-seedling pots. Samples of bio-seedling pots were created, containing similar chemicals but with varying cellulose concentrations (5wt%, 10wt%, 15wt%). Each cellulose concentration was replicated three times and cross-linked with different citric acid concentrations (40wt%, 45wt%,50wt%). The tensile strength (TS), compressive strength (CS), water solubility, and biodegradability of all samples were tested, revealing improved performance with higher component concentration. The sample with the highest component ratio exhibited promising durability (TS: 42N; CS:295N), biodegradability rate (12.5%/day), and water solubility rate (25%/day), supporting healthy plant growth and demonstrating significant performance under varying production conditions. The results conclude that cellulose from Malunggay branches has the potential to be an effective, sustainable material for bio-seedling pot production. This contributes to more environmentally friendly agricultural practices and offers insights into eco-friendly innovations in agriculture.